Molecular Detection and Partial Characterization of Strawberry Crinkle Virus in Field-Grown Strawberry Plants

Abstract

Molecular detection and partial characterization of Strawberry crinkle virus (SCV) in field-grown strawberry plants were conducted to confirm viral presence and assess its genetic relationship with reported isolates. Field surveys were carried out in commercial strawberry plantations showing typical symptoms such as leaf crinkling, chlorosis, stunting, reduced runner production, and poor fruit development. Symptomatic and asymptomatic leaf samples were collected and subjected to molecular diagnostics using RT-PCR targeting conserved genomic regions of SCV. Amplified products were further sequenced for partial genomic characterization and phylogenetic analysis. Results confirmed the presence of SCV in symptomatic strawberry plants, while asymptomatic samples showed no detectable infection, indicating a strong association between viral presence and symptom expression. Sequence analysis revealed high similarity with previously reported SCV isolates, with minor nucleotide variations suggesting limited genetic divergence and possible regional adaptation. Phylogenetic reconstruction grouped the detected isolate within established SCV clades, confirming its taxonomic identity and evolutionary relationship with global populations. The study also indicates potential involvement of aphid vectors in field transmission, contributing to localized spread and disease persistence in strawberry cultivation systems. These findings emphasize the importance of early molecular diagnosis for accurate detection and management of SCV in strawberry fields. The study supports the implementation of virus-free planting material, vector control strategies, and regular field monitoring to reduce yield losses associated with strawberry crinkle disease.